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Pak. J. Bot., 47(3): 1115-1120, 2015. |
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Updated: 23-06-15 | ||||
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IDENTIFICATION AND ANALYSIS OF AN EFFICIENT DICOT CONSTITUTIVE PROMOTER FROM TOMATO
SARDAR BACHA1, ASIA KHATOON1, MUHAMMAD ASIF1, JOSHUA YUAN2 AND AFTAB BASHIR1*
Abstract: The regulatory sequence of sucrose synthase (susy) was explored in HTGS and screened using various bioinformatics tools for promoter prediction and identification of functional regulatory motifs. Transcription start site (TSS) was predicted in the promoter sequence. Species specific motifs were identified by using Plant PAN database. The Plant Care predicted various light responsive, hormone inducible and tissue specific motifs in the full length promoter which may be essential for the constitutive expression governed by this promoter. Full length susy promoter isolated from tomato (Solanum lycopersicum) drove maximum transient expression of GUS gene in various tissues of tobacco, cotton and peas. The susy promoter identified and analyzed in this study is suitable for transgene expression in economically important agricultural crops, especially to avoid strong over-expression.
Key words: Dicot, Promoter, Sucrose synthase, Tomato, TSS
1 National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan2 Department of Plant Pathology and Microbiology, Texas A&M University, USA*Corresponding author’s e-mail: aftabb.pk@gmail.com |
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