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Pak. J. Bot., 47(3): 1153-1160, 2015. |
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Updated: 23-06-15 | ||||
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NATURAL INCIDENCE OF AFLATOXINS, MYCOLOGICAL PROFILE AND MOLECULAR CHARACTERIZATION OF AFLATOXIGENIC STRAINS IN CHICKPEA FLOUR
SAIRA MUSHTAQ1, ABIDA AKRAM1, NAFEESA QUDSIA HANIF2, RAHMATULLAH QURESHI1*, ZAHID AKRAM3, SHAISTA AKHUND1 AND BRIAN GAGOSH NAYYAR1
Abstract: The mycological profile of retail chickpea flour (locall called Baisan), sold in the markets in the Rawalpindi district was studied. All the samples were tested for the contamination with aflatoxins. A total of 13 fungal species isolated from the flour and out of which, Aspergillus flavus was recorded the most common species (100%), followed by Rhizopus oryzea (50%), Aspergillus niger (40%), Penicilium digitatum (30%), Cladosporium cladosporoides, Fusarium oxysporium, Mucor recemosus, M. petrinsularis and Rhizopus arrhizus (20% each), Aspergillus oryzea, Botritus cinerea, Mucor circineloides and Penicillium sp. (10% each). Aflatoxin B1 was found in only 20% of the samples ranging from 3.03-4.24ppb. The molecular characterization was carried out by using PCR using simple sequence repeats (SSR) primers. The SSR amplification pattern clearly showed the genetic variability among the 10 strains of A. flavus. A dendrogram was generated through MVSP software program. Genotype AF04 was most diverse among all genotypes. The similarity value was ranged between 0.538 (53.8%)-0.938 (93.8%).
Key words: Chickpea, Molecular characterization, Aflatoxins, Dendrogram, PCR, SSR.
1Department of Botany, Pir Mehr Ali Shah Arid Agriculture university Rawalpindi, Pakistan 2Romer Labs, Pakistan, Rawalpindi, Pakistan 3Department of Plant Breeding ad Genetics, PMAS-Arid Agriculture university Rawalpindi, Pakistan *Corresponding author: rahmatullahq@yahoo.com, rahmatullahq@uaar.edu.pk |
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