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Pak. J. Bot., 47(3): 1183-1189, 2015. |
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Updated: 23-06-15 | ||||
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MOLECULAR MAPPING OF STRIPE RUST RESISTANCE GENE YrSE5756 IN SYNTHETIC HEXAPLOID WHEAT AND ITS TRANSFER TO COMMON WHEAT
Y.J. WANG1, C.Y. WANG1, H. ZHANG1, M. ALI2, F. XUE3, X.L. LIU1, C.H. CHEN1 AND W.Q. JI1*
Abstract: Synthetic hexaploid wheat is an important germplasm resource for transfer of beneficial genes from alien species to common wheat (Triticum aestivum L.). Synthetic hexaploid wheat SE5756 confers a high level of resistance against a wide range of races of Puccinia striiformis West. f. sp. tritici Eriks. et Henn.(Pst). The objectives of this study were to determine the inheritance pattern, adjacent molecular markers, and chromosomal location of the stripe rust resistance gene in SE5756 and to develop new germplasm. We constructed a segregating population of 116 F2 plants and corresponding F2:3 families from a cross between SE5756 and Xinong979 with Pst races CYR32. Genetic analysis revealed that a single dominant gene, tentatively designated as YrSE5756, was responsible for seedling stage stripe rust resistance in SE5756. A genetic map, encompassing Xwmc626, Xwmc269, Xgwm11, Xbarx137, Xwmc419, Xwmc85, Xgpw5237, Xwmc134, WE173, Xwmc631, and YrSE5756, spanned 70.1 cM on chromosome 1BS. Xwmc419 and Xwmc85 were flanking markers tightly linked to YrSE5756 at genetic distances of 2.3 and 1.8 cM. Typical adult plant responses of the SE5756, varieties of the carrier Yr10 and Yr15, Chuanmai 42 (Yr24/Yr26), Yuanfeng 175 (Yr24/Yr26) and Huixianhong resistant to mixture Pst races (CYR32, CYR33 and V26) were experimented. The results showed that YrSE5756 was likely a new resistance stripe rust gene different from Yr24/Yr26, Yr10 and Yr15. From cross and backcross populations of SE5756/Xinong 979, we developed four new wheat lines with large seeds, stripe rust resistance, and improved agronomic traits: N07178-1, N07178-2, N08256-1, and N08256-2. These new germplasm lines could serve as sources of resistance to stripe rust in wheat breeding. SE5756 has the very vital significance in the development of breeding and expand our resistance germplasm resource gene pool.
Key words: Synthetic hexaploid wheat; Stripe rust; Resistance gene; Genetic mapping; Germplasm utilization.
1 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, 712100 Yangling, Shaanxi, China2 Department of Biotechnology, Sindh Agricultural University, Tandojam Sindh, Pakistan3 College of Agronomy, Shihezi University, 832000 Shihezi, Xinjiang, China*Corresponding author’s e-mail: jiwanquan2008@126.com; Phone: +86-29-87081319; Fax: +86-29-87081319 |
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