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Pak. J. Bot., 48(1): 131-135, 2016. |
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Updated: 29-02-16 | ||||
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MALE SPECIFIC GENE EXPRESSION IN DIOECIOUS PHOENIX DACTYLIFERA (DATE PALM) TREE AT FLOWERING STAGE
ABDULHAFED A. AL-AMERI, FAHAD AL-QURAINY, ABDEL-RHMAN Z. GAAFAR, SALIM KHAN* AND M. NADEEM
Abstract: Date palm is a long-living and evergreen important tree in the semiarid regions. Its fruit is rich in carbohydrate and fibres. Transcriptional profiling was compared among male and female trees of dioecious date palm at flowering stage. Male specific genes are expressed at flowering stage which was studied using the cDNA–SCoT marker. We developed sequence characterized amplified region (SCAR) markers of size 253 bp from male tree based on cDNA-SCoT fingerprinting. Further, developed SCAR marker was validated on the independently collected samples of both types of trees at flowering stage. The unique and specific band (253 bp) was amplified from male samples only whereas it was absent from female samples.
Key words: SCAR, cDNA, SCoT, Transcriptional profiling, Sequencing.
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia *Corresponding author’s email: salimkhan17@yahoo.co.in; Tel.: +966-014675865; Fax: +966-4678301 |
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