|
Pak. J. Bot., 48(1): 277-283, 2016. |
Back to Contents | ||||
|
|
Updated: 29-02-16 | ||||
|
|
EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF LEAVES, FRUIT AND BARK OF KIGELIA AFRICANA
TANVEER HUSSAIN2 , IRAM FATIMA1*, MUHAMMAD RAFAY2*, SAMMIA SHABIR1, MUHAMMAD AKRAM3 AND SABAHAT BANO1
Abstract: In vitro antibacterial activity of extracts was tested against six bacterial strains viz. Staphylococcus aureus, Proteus vulgaris, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Citrobacter amalonaticus by agar-disc diffusion method. Ethanol and n-hexane were used as negative control and oxytetracycline was used as a positive control. Ethanolic and aqueous extracts of bark and leaves of Kigelia africana showed remarkable activity against various bacterial strains as compared to n-hexane. S. aureus and E. coli were proved as highly sensitive strains while K. pneumonia was the resistant strain as the extracts formed no inhibition zone against it. The percentage of antioxidant activity of different parts of Kigelia was assessed by DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical assay. Quercetin was used as a standard antioxidant which showed 93.6% inhibition. Kigelia bark extract showed good antioxidant activity i.e., 67.33% inhibition, fruit extract possess moderate antioxidant activity i.e., 62.66% inhibition while leaves showed the poor antioxidant activity i.e., 59.66% DPPH inhibition respectively. Overall, the comparative analysis revealed that bark extract exhibited the most remarkable antibacterial as well as antioxidant activity as compared to leaves and fruit extracts.
Key words: Kigelia africana, Bacterial strains, Disc-diffusion method and DPPH-radical scavenging assay.
1Department of Life Sciences, The Islamia University of Bahawalpur, Pakistan 2Department of Forestry, Range and Wild Life Management, UCA&ES, The Islamia University of Bahawalpur, Pakistan 3 COMSATS Institute of Information Technology, Vehari, Pakistan *Corresponding author E-mail: Iramfatima46@yahoo.com,rafay@iub.edu.pk |
||||
|
|
|||||
| Back to Contents |
|
Back to Contents | |||
|
|
|||||