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Pak. J. Bot., 48(1): 285-290, 2016.

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ESTABLISHMENT OF AN EFFICIENT AND REPRODUCIBLE REGENERATION SYSTEM FOR POTATO CULTIVARS GROWN IN PAKISTAN

AAMIR IQBAL^12*, ALI RIZWAN¹², ZAHID MUKHTAR¹², SHAHID MANSOOR¹², ZAFAR MEHMOOD KHALID³ AND SHAHEEN ASAD¹²

Abstract: The present study was carried out to assess the effect of growth regulators in three different combinations on mass propagation of currently grown three potato cultivars cv. Desiree, Kuroda and Cardinal. Varietal response on In vitro regeneration under different hormonal combinations. For callus induction, internodes of potato cultivars were cultured on modified Murashige & Skoog (MS) medium, added with different growth hormonal combinations. Callusing frequency for all these treatments and cultivars were recorded and in callus induction medium (CIM1) explants showed significantly higher callus formation as compared to two other combinations. For shoot induction, calli were cultured on Murashige & Skoog (MS) modified medium, supplemented with different hormonal combinations. Shoot induction medium (SIM3) gave best shoot induction frequency as compared to other media combinations. On the same media, an average number of shoots per explant were obtained for cultivar Desiree which is significantly different from the other two media combinations. Overall, the In vitro regeneration and multiplication potential was highest in the variety Cardinal followed by Kuroda and Desiree. The interaction between different hormonal combinations and varietal response for all the parameters showed significant differences.

Ky words: Potato; Regeneration; Tissue culture; Micropropagation.

Abbreviations: 2, 4-D: 2, 4-Dichlorophenoxyacetic acid, NAA: 1-naphthaleneacetic acid, BAP: 6-benzylaminopurine, ZR: Zeatin riboside, IAA: Indole-3-acetic acid, IBA: Indole-3-butyric acid.

¹Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Jhang Road, Faisalabad, Pakistan

²Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan

³Department of Biotechnology and Bioinformatics, International Islamic university, Islamabad, Pakistan

^*Corresponding author’s e-mail: aftab6104@gmail.com