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Pak. J. Bot., 48(1): 305-309, 2016. |
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Updated: 29-02-16 | ||||
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OPTIMIZING THE RAPID TECHNIQUE FOR PROPAGATION OF CERASUS CAMPANULATA BY TISSUE CULTURE
BIHUA CHEN1,2*, JIANMIN LI1,2, JUAN ZHANG1,2, ZHUOXI WU1,2, HUIHUA FAN1,2 AND QIANZHEN LI1,2
Abstract: The initiation medium for Cerasus campanulata was 1/2MS+1.0 mg L-1 BA+0.1 mg L-1 NAA +30 g L-1 sugar, and the average initiation rate was 94.4%. The proliferation medium composed of MS+0.3 mg L-1 BA+0.2 mg L-1 NAA+30 g L-1 sugar provided proliferation rate 3.6 and average shoot length 2.3 cm. The rooting medium composed of 1/2MS+0.6 mg L-1 IBA+20 g L-1 sugar provided rooting rate 85.6%, average root number per individual 5.9 roots, and average root length 2.7 cm. The dishwashing detergent instead of Tween-20 as the spreader reduced the contamination rate for the explant disinfection. The red-core soil adopted to be the plantlet transfer medium provided 91.3% survival rate. The light media LZ and SK were not eligible to be the transfer medium for the species.
Key words: Cerasus campanulata (Maxim.) Yu et Li, Tissue culture; Medium, Dishwashing detergent; red-core soil.
1Fujian Academy of Forestry Sciences, Fuzhou 350012, China; 2Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, China Forestry Bureau, Fuzhou 350012, China *Corresponding author’s e-mail: chenbihua.happy@163.com |
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