HIGHLIGHTING THE MECHANISMS BY WHICH PROLINE CAN CONFER TOLERANCE TO SALT STRESS IN CAKILE MARITIMA

 

DORSAF MESSEDI^1*, FETEN FARHANI¹, KARIM BEN HAMED¹, NAJLA TRABELSI¹, RIADH KSOURI¹, HABIB-UR-REHMAN ATHAR², CHEDLY ABDELLY¹

 

Abstract: Cakile maritima is an oleaginous halophyte growing in the sandy dunes along the Tunisian coast. In order to investigate the role of proline in inducing high salinity tolerance (200 and 400 mM NaCl) in this halophyte, we studied several aspects of the salt responses of C. maritma under exogenous proline supply (20 mM). Salinity levels above 100 mM, reduced growth, photosynthetic activity, and quantum yield of photosystem II (FPSII), while increasing the non photochemical quenching (NPQ). Significant inhibition of the linear electron transport rate (ETR) was also observed in plants grown at 400 mM NaCl. In addition, polyphenol content, total antioxidant and DPPH scavenging activities increased due to increasing salinity stress, and the concentration of malondialdehyde (MDA) also increased. The application of proline counteracted all these adverse effects of salt stress in plants grown at 200 mM NaCl, while it improved some of these physiological attributes at 400 mM NaCl. In addition, contribution of Na⁺ for the osmotic adjustment decreased in the leaves of salt treated plants supplied with proline exogenously. Exogenous application of proline induced the accumulation of potassium, proline and soluble carbohydrates in salt stressed plants, particularly at 400 mM. This explained the reason of growth enhancement induced by proline application. All together, our results showed that the beneficial effect of exogenous proline on the response of C. maritima to salinity was due to its role in the protection of chloroplast structures, antioxidant defenses and osmotic adjustment.

 

Key words: Cakile martima; Exogenous proline; Osmoregulation; Osmoprotection; Induced tolerance; Salt stress.