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Pak. J. Bot., 48(2): 589-593, 2016. |
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Updated: 01-04-16 | ||||
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IN-VITRO REGENERATION OF CALENDULA MARITIMA GUSS. (ASTERACEAE), A THREATENED PLANT ENDEMIC TO WESTERN SICILY
CARRA ANGELA1, BAMBINA MARCELLA2, PASTA SALVATORE1, GARFÌ GIUSEPPE1, BADALAMENTI ORNELLA1, CATALANO CATERINA1, CARIMI FRANCESCO1* AND SAJEVA MAURIZIO2
Abstract: Calendula maritima is a critically endangered endemic plant of Western Sicily. Besides habitat destruction, the hybridization with the contiguous congener species C. fulgida is a major threat to its conservation. For this reason, seed-based propagation and seed storage are not appropriate for conservation purposes. In the present paper we describe a rapid and prolific In vitro plant regeneration method by direct organogenesis from leaves of C. maritima. Leaf explants were cultured on solid Murashige and Skoog (MS) medium in the presence of several plant growth regulator combinations. The best shoot multiplication rate (2.5 shoots/explant) was obtained on the medium containing 4.4 µM 6-benzylaminopurine in combination with 10 µM ß-naphthoxyacetic acid. Regenerated shoots were successfully rooted on solid MS medium supplemented with several auxins and the best result was obtained with 1.0 µM indole-3-acetic acid (35% of plantlets rooted). Plantlets were thereafter established in the greenhouse (survival frequency 75%) and no phenotypic variations were observed between regenerants and the mother plants.
Key words: Endangered species, Ex situ conservation, Organogenesis, Plant regeneration.
Abbreviations: BAP, 6-benzylaminopurine; IAA, Indole-3-acetic acid; IBA, Indole-3-butyric acid; NOA, ß-naphthoxyacetic acid; PGR, plant growth regulators; TDZ, thidiazuron
1Institute of Biosciences and BioResources (IBBR), National Research Council (CNR), Research Division of Palermo, Corso Calatafimi 414, 90129 Palermo, Italy 2Department STEBICEF, University of Palermo, via Archirafi 18, 90123 Palermo, Italy *Corresponding author’s e-mail: francesco.carimi@ibbr.cnr.it; Phone: +39-091-6574578; Fax: +39-091-423424 |
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