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  Pak. J. Bot., 48(2): 673-680, 2016.

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  Updated: 01-04-16

 

 

CLONING AND EXPRESSION STUDY OF BNALCR78 IN BRASSICA NAPUS

 

LI ZHUANG1*, LIU YING ZE1, WU YONG CHENG1, GUO SHI XING1, HOU KAI1, DU JUN BO1 AND LIN LI LI2

 

Abstract: BnaLCR78 genes of three types of rape were cloned in rape (Brassica napus), and encoded protein structure was analyzed, the results showed that the protein had a conserved coding domain which was analogues among LCR family of Arabidopsis. The expression patterns of genes of three types of rape in varying tissues and in specific same tissues were analyzed using quantitative method. The results showed that their expression patterns differ from that of former research in Brassica napus, which may result from the difference of sampling time. We speculated that the gene might be involved in transpiration and transportation and distribution of nutrient, oil content in seed.

 

Key words: Cloning, BnaLCR78, Brassica napus, Brassica campestris, and Brassica juncea.
 


1College of Agriculture, Si-Chuan Agricultural University, Cheng Du, P.R. China

2College of Environment, Si-Chuan Agricultural University, Cheng Du, P.R. China

* Corresponding author’s email: lizhuang2012@sicau.edu.cn; Tel: +86 18380285881


   
   

 

   
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