Pak. J. Bot., 48(2): 717-722, 2016.

EFFECT OF GENOTYPE AND INFLORESCENCE DEVELOPMENTAL STAGE ON CALLUS INDUCTION AND PLANT REGENERATION OF MISCANTHUS LUTARIORIPARIU, A NON-FOOD BIOMASS CROP

LINGLING ZHAO1, YING DIAO1, SURONG JIN2, FASONG ZHOU1 AND ZHONGLI HU1*

Abstract: Miscanthus lutarioriparius, is a perennial C4 weeds. In this study, tissue culture responses were evaluated in four genotyoes of M. lutarioriparius which collected from the Yangtze River basin. A total of four types of callus with different characteristics and induction frequencies were induced from four genotypes immature inflorescences on medium Murashige and Skoog (MS) containing 4.0 mg/L 2,4-dichlorophenoxyacetic acid (2, 4-D). Only ML04 generated the embryogenic callus. According to the length of immature inflorescences, they divided into three inflorescences developmental stages which generated different induction percentages of embryogenic callus. Developmental stage B with 50-100 mm length inflorescence generated the highest induction percentages of embryogenic callus. The regeneration rate was 65.56% (4.0 mg/L kinetin), 91.50% (5.0 mg/L KT) and 26.67% (6.0 mg/L KT) from embryogenic callus, respectively. The shoots were transferred onto the Murashige and Skoog (MS) basal medium for rooting, the rooting rate was 100%.

Key words: Micanthus lutarioripariu, Inflorescences developmental stage, Embryogenic callus, Plant regeneration.

1State Key Laboratory of Hybrid Rice, College of Life Science, Wuhan University, Wuhan, Hubei 430072, China

2School of Science, Wuhan University of Technology, Wuhan, Hubei 430070, China

*Corresponding author’s email: huzhongli@whu.edu.cn; Tel: 86 27 68753611 Fax: 86 27 68753611