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Pak. J. Bot., 48(2): 779-791, 2016.

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MICROBIAL DIVERSITY AND METAGENOMIC ANALYSIS OF THE RHIZOSPHERE OF PARA GRASS (UROCHLOAMUTICA) GROWING UNDER SALINE CONDITIONS

SALMA MUKHTAR¹, MUHAMMAD S. MIRZA², HUMERA A. AWAN¹, ASMA MAQBOOL¹, SAMINA MEHNAZ¹ AND KAUSER A. MALIK^1*

Abstract: Para grass is a salt tolerant plant, grown on salt affected soils of Punjab, Pakistan. The aim of this study was to investigate the distribution of culturable and non-culturable bacteria in the rhizosphere, rhizoplane and histoplane of para grass, growing under saline conditions. A total of seventy four, bacterial strains were isolated and characterized. Among these, thirty two from rhizosphere, twenty two from rhizoplane and twenty were from the histoplane. Cultureable bacteria were characterized by biochemical tests and 16S rRNA gene sequence analysis. Non-culturable bacteria were identified by PCR amplification of 16S rRNA gene, using metagenomic approach. Seventy seven percent bacterial isolates from rhizosphere and rhizoplane fractions were identified as member of Proteobacteria. Twenty five percent isolates of histoplane fraction were members of firmicutes while 68.75% were of Proteobacteria. Of total isolates, 50% could grow in nitrogen free medium and 21.67% on halophilic medium. Nitrogen fixers and halophilic bacteria were more abundant in the rhizosphere as compared to roots. 16S rRNA gene clone library analysis showed that out of 48 clones, 14 were uncultured, classified; eighteen un-cultured un-classified, while others related to 16 different known cultured groups of bacteria. Results for cultured and uncultured bacteria revealed a wide diversity of bacterial population present in the rhizosphere of para grass.

Key words: Halophilic bacteria, 16S rRNA gene, Uncultureable bacteria, Metagenomics, Para grass.

¹Department of Biological Sciences, Forman Christian College (A Chartered University), Ferozepur Road, Lahore 54600, Pakistan

²Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), JhangRaod, Faisalabad, Pakistan

Corresponding author’s email: kausermalik@fccollege.edu.pk