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EFFECTS OF PLANT GROWTH REGULATORS ON SEED GERMINATION AND CALLUS
INDUCTION OF HYLOCEREUS COSTARICENSIS
WINSON KOE WEI SHENG1, JEEVANDRAN SUNDARASEKAR2,
KATHIRESAN SATHASIVAM1 AND SREERAMANAN SUBRAMANIAM3*
Abstract:
Dragon fruit (Hylocereus costaricensis) belongs to the family Cactaceae
and are climbing vines which have received worldwide attention in recent
years. However, there are still lack of information on the protocols for
the establishment of In vitro culture system. In the present study, seed
germination percentage were determined by culturing seeds on semi-solid
Murashige and Skoog medium (MS) supplemented with 1 ppm
6-Benzylaminopurine (BAP) together with either 0, 0.5 or 0.8 ppm of
Indole-3-butyric acid (IBA). Germination percentage was the highest by
using plant growth regulators (PGRs) combination of 1 ppm BAP and 0 ppm
IBA (93.33%). Subsequently, the cotyledons from seedlings of the
germinated seeds were used for subsequent callus induction. Small pieces
of cotyledons were excised and cultured on MS medium fortified with
0.45, 0.9, 1.8, 2.7, 3.6, and 4.5 ppm of 2,4-Dichlorophenoxyacetic acid
(2,4-D) together with either 0, 0.9 or 1.8 ppm of BAP. Callus induction
percentage was highest using the plant growth regulators (PGRs)
combination of 3.6 ppm 2,4-D and 1.8 ppm BAP (75%). Hence, 3.6 ppm of
2,4-D and 1.8 ppm BAP was the best combination for callus induction of
Hylocereus costaricensis.
Key words: Seed germination, Callus, Hylocereus costaricensis,
1Department of Biotechnology, Faculty of Applied Sciences, AIMST
University, Semeling, 08100 Bedong, Kedah Darul Aman, Malaysia 2School
of General and Foundation Studies, AIMST University, Semeling, 08100
Bedong, Kedah Darul Aman, Malaysia; 3School of Biological Sciences,
Universiti Sains Malaysia, Minden Heights, 11800 Penang, Malaysia
*Corresponding author's e-mail: sreeramanan@usm.my /
sreeramanan@gmail.com
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