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Pak. J. Bot., 48(3): 1133-1137, 2016.

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QTL Mapping of Adult-Plant Resistance to Leaf Rust in Chinese wheat Cultivar Lantian 9

Han Liusha^1√, Li Zaifeng^1√, Wang XIAODONG¹, KANG ZHANHAI^1,2, Zhu Lin¹, Li Xing^1,2*, Liu Daqun^1* AND Syed Jawad Ahmad Shah²

Abstract: Chinese wheat cultivar “Lantian 9” showed a stable high yield in the field. Together with its other desirable traits such as tolerance to cold, drought, leaf rust and stripe rust, made it a good source for wheat breading. In our preliminary test, “Lantian 9” showed a typical adult resistance phenotype (susceptible at seedling stage but high resistance at adult stage) to most of the major Chinese leaf rust pathotypes. To clarify the adult-plant resistance (APR) genes in this cultivar, a population with 197 F_2:3 lines was generated by crossing “Lantian 9” with susceptible line “Huixian Hong”. The population was phenotyped in the field over three years (year 2012, 2013 and 2014) by a mixture of three leaf rust pathotypes (THTT, THTS and THTQ). A total of 1232 simple sequence repeat (SSR) markers were used to screen the parental lines. Polymorphic ones were further applied on the population. Linkage mapping analysis showed that one QTL from “Lantian 9” was located on chromosome 2BS, which was relative stable among the data from the year 2012 and 2013 with phenotypic variations of 6.0% and 9.1%, respectively. Three other QTLs from “Lantian 9” on chromosome 4BS, 3A and 1BL were detected. We also identified one QTL from “Huixian Hong” on chromosome 1BL. All these identified wheat leaf rust resistance QTLs with their closely linked molecular markers will greatly facilitate genetic improvement of wheat resistance to leaf rust in China.

Key words: Wheat, Leaf rust, Resistance gene, QTL mapping.
 

¹College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei, Baoding 071001, People’s Republic of China

²State Key Laboratory for Biology of Plant Diseases and Insect Pests, Beijing 100193, People’s Republic of China

³NIFA Tarnab, Peshawar 25000, Pakistan

^*Corresponding author’s email: lixing@hebau.edu.cn; ldq@hebau.edu.cn; Tel:+86-312-7528500

^√These two authors have contributed equally to this study