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Pak. J. Bot., 48(3): 1145-1151, 2016. |
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Updated: 16-06-16 | ||||
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EVALUATION OF POLYPHENOL CONTENT IN DIFFERENT PARTS OF PHYSALIS IXOCARPA
WAJID KHAN1,2 JEHAN BAKHT1* AND MOHAMMAD SHAFI3
Abstract: In the current study extracts of leaf, stem, fruit and calyx with different polarity was investigated for their phenolic content using high performance liquid chromatography and spectrophotometric assay. Among different parts, stem contain high concentration of total polyphenol and gallic acid. The effect of extraction solvent on polyphenol quantification was observed in both assays. Spectrophotometric analysis of the data regarding polyphenol content indicated that among different extracts from the stem, leaf and fruit tissues; ethyl acetate extracted fraction of stem measured maximum polyphenol content of 110.376 mgGAE/g of dry extract. The ethyl acetate extracted sample of leaf showed high polyphenol (Gallic acid) content of 95 mg GAE/g of dry extract using high performance liquid chromatography assay. The amounts of phenolic content (Gallic acid) extracted from the parts of the plant with the different solvent ranged from 0.0354- 95 mg GAE/g of the dry extract using HPLC, however, spectrophotometric assay indicated total polyphenol ranged from 38-110.37 mgGAE g-1 of the dry extract. The current study suggested that ethyl acetate is an effective solvent for the extraction of polyphenol in different parts of P. ixocarapa.
Key words: Polyphenol; Folin–Ciocalteu reagent;
Gallic acid equivalent; Spectrophotometric assay; P. ixocarapa 1Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar KPK Pakistan 2Center of Biotechnology and Microbiology, University of Swat KPK Pakistan 3Department of Agronomy, The University of Agriculture Peshawar KPK Pakistan *Corresponding author’s email: jehanbakht@yahoo.co.uk |
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