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Pak. J. Bot., 48(3): 1153-1158, 2016.

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MICROPROPAGATION OF GINGER (ZINGIBER OFFICINALE VAR. RUBRUM) USING BUDS FROM MICROSHOOTS

ZURAIDA A.R.^1*,  MOHD SHUKRI M.A.², ERNY SABRINA M.N.³, AYU NAZREENA O¹ , CHE RADZIAH C. Z.⁴, PAVALLEKOODI G.⁵ AND SREERAMANAN S.⁵

Abstract: Zingiber officinale var. Rubrum (ZOR) is cultivated for its medicinal value despite the constraints of longer life cycle. The study has established an efficient and reproducible protocol to micropropagate ZOR using buds generated on the surface of the ginger. Surface sterilized young buds of 0.5-1 cm and 2-4 cm cultured on Murashige and Skoog (MS) supplemented with BAP showed the highest survival rate (55-65%) and produced the highest average number of microshoots per explant (3.2±0.06) respectively. MS medium supplemented with different concentrations and combinations of auxin and cytokinin were used to evaluate shoot multiplication and root induction. BAP concentrations between  3.0-5.0 mg/L was very effective in promoting microshoots and resulted in 100% of microshoot propagation. Microshoots cultured on MS medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA produced the highest number of shoots while 0-0.5 mg/L BAP enhanced shoot length and 3mg/L NAA in combination with BAP produced highest number of roots. Microshoots maintained on MS medium supplemented with 4.5% sucrose produced the highest number of plantlets (23±2.5) and roots per explants (15.4±2.4) meanwhile reducing the length of lateral roots (2.6±0.2).

Key words: Zingiber officinale, Micropropagation, Plant growth regulator, Carbon source, Young bud
 

¹Biotechnology Research Centre, MARDI Headquarters, MARDI HQ, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia

²Genebank and seed centre, MARDI HQ, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia

³Agrobiodiversity & Environment Research Centre, MARDI HQ, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia ⁴Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia.

⁵School of Biological Sciences, Universiti Sains Malaysia, Georgetown, 11800, Penang, Malaysia

^*Corresponding authors’ e-mail: azuraida@mardi.gov.my