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Pak. J. Bot., 48(3): 1189-1195, 2016. |
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Updated: 16-06-16 | ||||
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ALLELOPATHIC ACTIVITY OF LEONURUS SIRIBICUS L. ON SEED GERMINATION AND SEEDLING GROWTH OF WHEAT AND IDENTIFICATION OF 4- HYDROXY BENZOIC ACID AS AN ALLELOCHEMICAL BY CHROMATOGRAPHY
M.A. SAYED1, 2*, RAHSED IMAM1, M.N. SIDDIQUI3, S.M. RAIHANUN-NABI4, S. AKTAR1, AND S.R. DAS1
Abstract: The aim of this study was to investigate the allelopathic effects of L. siribicus extract on seed germination and seedlings growth of wheat as well as to identify potential allelochemical. The different concentration (5, 10 and 15%) of aqueous extract were applied during the time of sowing and at 5 days after sowing of wheat seed. L. siribicus extract showed concentration and time - depending activity. Different concentration of aqueous extract inhibited seed germination, seedlings growth, when extracts were applied during the time of seed sowing. The stimulatory effect of seedlings growth were found for 5 % aqueous extract, in contrast 10 and 15% extract inhibited seedlings growth, when extracts were applied at 5 days after sowing. Apart from, 4-hydroxy benzoic acids affected seedlings growth irrespective of application time. The weight of dry matter of wheat seedlings were increased for 5% than 10 and 15% extracts. Thin layer chromatography suggested that the presence of 4-hydroxy benzoic acid including other allelopathic and growth regulatory compounds inhibited germination and seedlings growth. Mineral composition was determined and its might have some stimulatory effect on seedlings growth. It was interesting that 5% extract inhibited germination and seedlings growth, when it was applied during the time of seed sowing, but stimulated seedling growth, when it was applied at 5 days after sowing. The extract of this plants can be used either for bioherbicide as well as growth stimulatory agents for the organic farming system. To find out molecular mechanism behind it, further research is to be done.
Key words: Allelopathic, Leonurus siribicus, Germination, Wheat, Chromatography.
1Department of Biochemistry and Molecular Biology, Hajee Mohammad Danesh Sceinece and Technology University, Dinajpur-5200, Bangladesh 2The United Graduate School of Agriculture, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550 Japan 3Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh 4Department of Biotechnology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh *Corresponding author’s email: sayed_bmb@yahoo.com |
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