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ASSESSMENT OF FUNCTIONAL TRAITS IN THE ASSEMBLAGE OF ENDOPHYTIC FUNGI OF
ANACARDIUM OTHONIANUM RIZZINI
PAULA SPEROTTO ALBERTO FARIA1, JAQUELINE ALVES SENÁBIO2, MARCOS ANTÔNIO
SOARES3, FABIANO GUIMARÃES SILVA4, ANA PAULA ALVES CUNHA5 AND EDSON LUIZ
SOUCHIE6
Abstract: Plants maintain symbiotic relationships with microorganisms as a
strategy to withstand adversities. From this exchange, organisms receive
photoassimilates and provide benefits to the plant. Anacardium
othonianum Rizzini, locally known as ‘caju-de-árvore-do-cerrado’ (tree
cashew ofthe cerrado), is a tree species of the family Anacardiaceae
nativeto the Midwest region of Brazil. The objective of this study was
to characterize the culturable endophytic fungal community, its
functional traits and its association with the roots of A. othonianum.
The roots of A. othonianum were fragmented (1 cm) and inoculated in
medium for the isolation of endophytic microorganisms. The molecular
identification of the isolates was performed through the partial
sequencing of the internal transcribed spacer (ITS). The endophytic
isolates were tested for the synthesis of indole acetic acid (IAA) and
phosphate solubilization through the colorimetric method.The root
fragments were cleared, stained and examined under a microscope.
Structures characteristic of endomycorrhizal and endophytic
microorganisms were found on the slides analyzed. A total of 67 fungal
strains were isolated and identified in 12 species: Fusarium oxysporum,
Bionectria ochroleuca, Periconia macrospinosa, Phomopsis lagerstroemiae,
Penicillium kloeckeri, Eupenicillium shearii, Phomopsis asparagi,
Penicillium pinophilum, Agaricomycetes sp., Diaporthe sp., Cladosporium
cladosporioide sand Paecilomyces lilacinus. All the genera found have
been reported in the literature as endophytic species. It can be
concluded that A. othonianum maintains associations with endomycorrhizal
and endophytic fungi. Twelve endophytic strains were isolated from A.
othonianum Rizzini, seven of which have potential for phosphate
solubilization and IAA synthesis.
Key words: Endophytic, Endomycorrhizal, Fungi, Anacardium othonianum,
Anacardiaceae,
1Master of Agricultural Science, Instituto Federal Goiano Câmpus Rio
Verde, Goiás, Brazil, CEP: 75901-970
2BIC Student, Universidade Federal do Mato Grosso, Cuiabá, Mato Grosso,
Brazil, CEP: 78060-900
3Prof Dr Universidade do Mato Grosso, Cuiabá, Mato Grosso, Brazil, CEP:
78060-900
4Prof Dr Instituto Federal Goiano Rio Verde, Goiás, Brazil, CEP:
75901-970
5IC Student, Instituto Federal Goiano Rio Verde, Goiás, Brazil, CEP:
75901-97
*Corresponding author’s email: paulasperotto@gmail.com
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