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Pak. J. Bot., 48(3): 1253-1262, 2016.

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CHARACTERIZATION OF SULFUR-OXIDIZING BACTERIA ISOLATED FROM ACID MINE DRAINAGE AND BLACK SHALE SAMPLES

WASIM SAJJAD¹, TARIQ MEHMOOD BHATTI², FARIHA HASAN¹, SAMIULLAH KHAN¹, MALIK BADSHAH¹, ABBAS ALI NASEEM³ AND AAMER ALI SHAH^1*

Abstract: Acid mine drainage (AMD) and black shale (BS) are the main habitats of sulfur-oxidizing bacteria. The aim of this study was to isolate and characterize sulfur-oxidizing bacteria from extreme acidic habitats (AMD and BS). Concentration of metals in samples from AMD and BS varied significantly from the reference samples and exceeded the acceptable limits set by the Environmental Protection Agency (EPA) and the World Health Organization (WHO). A total of 24 bacteria were isolated from these samples that were characterized both morphologically as well as through biochemical tests. All the bacteria were gram-negative rods that could efficiently oxidize sulfur into sulfate ions (SO₄^-2), resulted into decrease in pH up to 1.0 when grown in thiosulfate medium with initial pH 4.0. Out of 24, only 06 isolates were selected for phylogenetic analysis through 16S rRNA sequencing, on the basis of maximum sulfur-oxidizing efficiency. The isolates were identified as the species from different genera such as Alcaligenes, Pseudomonas, Bordetella, and Stenotrophomonas on the basis of maximum similarity index. The concentration of sulfate ions produced was estimated in the range of 179-272 mg/L. These acidophiles might have various potential applications such as biological leaching of metals from low-grade ores, alkali soil reclamation and to minimize the use of chemical S-fertilizers and minimize environmental pollution.

Key words: Sulfur-oxidizers, Acid mine drainage, Black shale, Alcaligenes, Pseudomonas, Bordetella, Stenotrophomonas
 

¹Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, 45320, Pakistan

²Department of Chemical Engineering, Pakistan Institute of Engineering and Applied Sciences, P.O. Nilore-45650, Islamabad, Pakistan,

³Department of Geology, Bacha Khan University, Charsada, Khyber Pukhtoonkhwa, Pakistan.

Corresponding author’s email: alishah@qau.edu.pk; Tel. No.: +92-51-90643116;