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Pak. J. Bot., 48(3): 1273-1277, 2016.

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GENETIC DIVERSITY OF MUNGBEAN GENOTYPES IN RELATION TO RESISTANCE AGAINST MUNGBEAN YELLOW MOSAIC VIRUS

RANA BINYAMIN^1*, MUHAMMAD ASLAM KHAN², AZEEM IQBAL KHAN³, FAISAL SAEED AWAN⁴, NASIR AHMAD KHAN² AND SOHAIL AKHTAR¹

Abstract: Mungbean Yellow Mosaic virus (MYMV) caused by single stranded DNA begomovirus is the most potential and prevailing threat to mungbean worldwide. This study was conducted to determine genetic variation in mungbean genotypes to know whether these genotypes, which differ in field screening results, also vary on a genetic basis or not. One hundred and twenty seven genotypes were screened against MYMV under the field condition. RAPD analysis showed significant amount of genetic diversity within mungbean germplasm. Twenty six primers produced 66% of polymorphism with average 5.36 bands per primer. Minimum similarity was observed among genotypes 8008 and Azri-06, which is 61.15%, while the maximum similarity was observed among genotypes NM-54 and NM-92, which is 91.37%. Three genotypes belonging to resistant category remained unclustered i.e., NM-2011, NM-2006 and Azri-06.Genetic differentiation among mungbean genotypes was similar to the field screening results. RAPD analysis revealed an extensive amount of variation, which could be used for cultivar identification. This information will be helpful in recognition of resistant germplasms and will be utilized for mungbean improvement program.

Key words: Genetic diversity, Mungbean yellow mosaic virus, RAPD
 

¹University of Agriculture Faisalabad Sub-Campus Burewala / Vehari

²Department of Plant Pathology, University of Agriculture, Faisalabad, Pakistan

³Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Pakistan

⁴Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture Faisalabad, Pakistan

^*Corresponding author’s email: binyamin1388@gmail.com