ROLE OF MUNGBEAN ROOT NODULE ASSOCIATED FLUORESCENT PSEUDOMONAS AND RHIZOBIA IN SUPPRESSING THE ROOT

ROTTING FUNGI AND ROOT KNOT NEMATODES IN

CHICKPEA (CICER ARIETINUM L.)

 

RUBINA NOREEN¹, ASMA SHAFIQUE¹, SYED ABID ALI², HABIBA³, VIQAR SULTANA⁴,

JEHAN ARA³ AND SYED EHTESHAMUL-HAQUE^1*

 

¹Department of Botany, University of Karachi, Karachi-75270, Pakistan

²HEJ Research Institute of Chemistry, University of Karachi, Karachi-75270, Pakistan

³Department of Food Science &Technology, University of Karachi, Karachi-75270, Pakistan

⁴Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan

^*Corresponding author’s email: ehtesham12@hotmail.com

 

Abstract

 

Three isolates each of fluorescent Pseudomonas (NAFP-19, NAFP-31 and NAFP-32) and rhizobia (NFB- 103, NFB-107 and NFB-109) which were originally isolated from root nodules of mungbean (Vigna radiata) showed significant biocontrol activity in the screen house and under field condition, against root rotting fungi viz., Macrophomina phaseolina, Fusarium  solani, F. oxysporum and  Rhizoctonia solani evaluated on chickpea. Biocontrol potential of these isolates was also evaluated against Meloidogyne incognita, the root knot nematode. Application of Pseudomonas and rhizobial  isolates as a soil drench, separately or mixed significantly reduced root rot disease under screen house and field conditions. Nematode’s penetration in roots was also found significantly less in rhizobia or Pseudomonas treatments used separately or mixed as compared to control. Fluorescent Pseudomonas treated plants produced greater number of nodules per plant than control plants and about equal to rhizobia treated plants, indicating that root nodule associated fluorescent Pseudomonas enhance root nodulation.

 

Key words: Chickpea, Rhizobia, Fluorescent Pseudomonas, Nodulation, Root rotting fungi, Nematode.