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Pak. J. Bot., 48(6): 2409-2422, 2016.

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ANALYSIS OF GENETIC DIVERSITY AMONG RAPESEED CULTIVARS AND BREEDING LINES BY SRAP AND SSR MOLECULAR MARKERS

SIRAJ AHMED CHANNA^1,2,†, HONGYUN TIAN^1,†, HONG QI WU¹ AND SHENG WU HU^1*

¹State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China

²Department of Plant Breeding and Genetics, Sindh Agriculture University Tandojam 70060, Sindh, Pakistan

^† Siraj Ahmed Channa and Hongyun Tian contributed equally to this work

^*Corresponding author’s e-mail address; swhu83251@nwsuaf.edu.cn, Ph: +862987082604

Abstract

The knowledge of genetic diversity is very important for developing new rapeseed (Brassica napus L.) cultivars. The genetic diversity among 77 rapeseed accessions, including 22 varieties and 55 advanced breeding lines were analyzed by 47 sequence-related amplified polymorphism (SRAP) and 56 simple sequence repeat (SSR) primers. A total of 270 SRAP and 194 SSR polymorphic fragments were detected with an average of 5.74 and 3.46 for SRAP and SSR primer, respectively. The cluster analysis grouped the 77 accessions into five major clusters. Cluster I contained spring and winter type varieties from Czech Republic and semi-winter varieties and their respective breeding lines from China. The 16 elite breeding lines discovered in Cluster II, III, IV and V indicated higher genetic distance than accessions in Cluster I. The principal component analysis and structure analysis exhibited similar results to the cluster analysis. Analysis of molecular variance revealed that genetic diversity of the selected breeding lines was comparable to the rapeseed varieties, and variation among varieties and lines was significant. The diverse and unique group of 16 elite breeding lines detected in this study can be utilized in the future breeding program as a source for development of commercial varieties with more desirable characters.