CONVENTIONAL BREEDING ENHANCES GENETIC DIVERSITY IN

PISUM SATIVUM (L.) DETECTED THROUGH SDS-PAGE

 

ASAF KHAN¹, ZABTA KHAN SHINWARI², AJMAL IQBAL¹,

SYED FAZAL WADOOD¹, NAUSHEEN¹, AND MOHAMMAD NISAR^1*

 

¹Department of Botany, University of Malakand, Chakdara Dir (L), KP-Pakistan

²Department of Biotechnology, Quaid-i-Azam, University, Islamabad, Pakistan

^*Corresponding author’s email: mnshaalpk@yahoo.com; Cell No: 03443-222200

 

Abstract

 

In the present study, twenty homozygous segregante of edible pea were developed through conventional breeding (2004- 2015). The seed storage proteins were extracted from the seeds of each line and run through Slab-vertical gel Electrophoresis. After staining and de-staining the Electrophoregram was divided into two zones (R-I and R-II). The R-1 was consists of five bands (B-1, 2, 3, 4 & 5) ranging from 180KDa to 34KDa, which calculated < 1% genetic diversity (GD). Out of which B-1, 2 and 3 were polymorphic, while B-4 and B-5 were monomorphic. Due to strong genetic association and low level of GD, the R-I divided the total germplasm into two clusters. Similarly, R-II was comprised of nine bands ranging from 33KDa -10KDa. All the loci were polymorphic and showed >39% genetic diversity. The R-II divided the total lines into four clusters. In cluster plating, 43.74% lines were grouped in C-03, 30.43% in C-01, 13.39% in C-04 and 8.69% in C-02. Variation in bands profile and multiple clustering authenticates that conventional plant breeding enhances the level of genetic variation in seed storage protein profile in pea germplasm detected through SDS-PAGE.

 

Key words: Peas homo-segregante, Biochemical markers, Diversity, Cluster analysis