IN VITRO EVALUATION OF MEDICINAL, ANTIOXIDANT ACTIVITIES AND

PHYTOCHEMICAL SCREENING OF IPHIONA GRANTIOIDES

AND PLUCHEA ARGUTA SUBSP. GLABRA QAISER

 

SHAHIDA NAVEED^1*, MUHAMMAD IBRAR² AND INAYATULLAH KHAN³

 

¹Department of Botany, Women University Swabi, Swabi, Pakistan

²Department of Botany, University of Peshawar, Peshawar, Pakistan

³Department of Agronomy, The University of Agriculture Peshawar, Pakistan

^*Corresponding author’s email: shahidanaveed@live.com

 

Abstract

 

The crude ethanolic extracts (EE) derived from different parts of Iphiona  grantioides (flower, leaf, stem and root) and Pluchea arguta subsp.glabra Qaiser  (leaf, stem and root) were screened In vitro for possible antioxidant, antibacterial, antifungal activities and phytochemical profile. Results for phytochemical screening revealed the presence of various important primary and secondary metabolites including carbohydrates, protein, essential oils, tannins, flavonoids, saponins, glycosides, phytosterol, tri-terphenoids and volatile oil in the ethanolic extracts (EE) of different parts of I. grantioides and P. arguta. Significant antibacterial activity was observed for flower and leaf (EE) of I. grantioides against Klebsiela pneumonia and Escherichia coli, while moderate antibacterial activity was showed by extracts from other parts of this plant. Similarly P. arguta leaf (EE) showed significant inhibitory activity against Escherichia coli, Pseudomonas aeroginosa and Micrococcus luteus than its stem and root’s extract. The crude ethanolic extract of all parts of I. grantioides and P. arguta proved a rich source of fungicidal potential. Both the plants proved to have antioxidant potential by exhibiting DPPH radical scavenging activity. So it may be concluded from the present investigations that I. grantioides and P. arguta, both have a wide range of antibacterial, antifungal and antioxidant properties and can be utilized for the development of antimicrobial drugs for human beings, animals and as safe pesticides in agricultural practices as well as could be an important source of natural radical scavengers.

 

Key words: Antibacterial, Antifungal, Antioxidant activity, Iphiona grantioides and Pluchea arguta subsp. glabra. Qaiser