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Pak. J. Bot., 49(1): 21-23, 2017.

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RESPONSE OF RICE POLYPHENOL OXIDASE PROMOTER TO

DROUGHT AND SALT STRESS

WASIM AKHTAR¹, AND TARIQ MAHMOO²*

¹Department of Biotechnology, University of Azad Jammu and Kashmir, Muzaffrabad-13100 (AJK), Pakistan

²Department of Plant Sciences, Quaid-i-Azam University, Islamabad-45320, Pakistan

*Corresponding author e-mail: tmahmood.qau@gmail.com;tmahmood@qau.edu.pk

Abstract

Polyphenol oxidases (PPO) widely exist in plants that catalyze oxygen dependent oxidation of phenols to quinines and assumed to be involved in plant defense against environmental stresses. In this study transgenic T1 seeds of Arabidopsis thaliana containing Oryza sativa Polyphenol oxidase (OsPPO) gene promoter fused with GUS (β-glucuronidase) were analyzed for drought and salt stress. These seeds were already available in our research group. Seeds were germinated on Murashige and Skoog (MS) mediato get T2 plants which were screened in drought stress (5, 10, 15, 20, 25 and 30% PEG-6000) and salt concentrations (50 mM, 100 mM, 150 mM, 200 mM, 250 mM and 300 mM). Experimental data showed that relative GUS expression of OsPPO gene promoter increased with the increase of drought stress. In case of salt stress, OsPPO induction showed similar trend GUS expression was increased. The response of OsPPO to drought and salt stress suggest the possible participation of PPO in plants defense against drought as well as salt stress.

Key words: Oryza sativa, PPO promoter, GUS, Arabidopsis thaliana, Salt and drought stress.