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Pak. J. Bot., 49(1): 125-132, 2017.

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ANALYSIS OF THE A GENOME GENETIC DIVERSITY AMONG BRASSICA NAPUS,

B. RAPA AND B. JUNCEA ACCESSIONS USING SPECIFIC

SIMPLE SEQUENCE REPEAT MARKERS

HONGYUN TIAN^1,†, JINQIANG YAN^1,†, SIRAJ AHMED CHANNA^1,2,†,

RUIJIE ZHANG¹ ,YUAN GUO¹AND SHENGWU HU^1★

¹State Key Laboratory of Crop Stress Biology in Arid Areas and College of Agronomy,

Northwest A&F University, Yangling, Shaanxi 712100, China

²Department of Plant Breeding and Genetics, Sindh Agriculture University Tandojam 70060, Sindh, Pakistan

†Hongyun Tian,Jingqiang Yan, and Siraj Ahmed Channa contributed equally to this work

^★Corresponding author’s e-mail address; swhu83251@nwsuaf.edu.cn, Ph: +862987082604

Abstract

This investigation was aimed at evaluating the genetic diversity of 127 accessions among Brassica napus, B. rapa, and B. juncea by using 15 pairs of the A genome specific simple sequence repeat primers. These 127 accessions could be clearly separated into three groups by cluster analysis, principal component analysis, and population structure analysis separately, and the results analyzed by the three methods were very similar. Group I comprised of mainly B. napus accessions and the most of B. juncea accessions formed Group II, Group III included nearly all of the B. rapa accessions. The result showed that 36.86% of the variance was due to significant differences among populations of species, indicated that abundance genetic diversity existed among the A genome of B. napus, B. rapa, and B. juncea accessions. B. napus, B. rapa, and B. juncea have the abundant genetic diversity in the A genome, and some elite genes can be used to broaden the genetic base of them, especially for B. napus, in future rapeseed breeding program.

Key words: Genetic diversity; Simple sequence repeat; A genome; Brassica cultivars.