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EXPRESSION AND THE SUBCELLULAR LOCALIZATION OF ATPUB19,

A U-BOX E3 LIGASE IN ARABIDOPSIS

YONG-CHANG LIU, HAO ZHANG, JIE-JIE LEI AND JIE SUN^*

College of Agriculture/The Key Laboratory of Oasis Eco-agriculture, Shihezi University,

Shihezi, Xinjiang 832003, China

^*Corresponding author's email: sunjiezh@yahoo.com.cn

Abstract

Plant U-box containing proteins (PUBs) belong to a new type of E3 ligase and have diverse roles in plant development and growth. In our previous study, we found that PUB19 negatively regulated the ABA-induced stomata closure, stress-related genes expression and drought tolerance. To obtain more information on PUB19, the expression pattern and subcellular localization were analyzed. Northern blot and GUS staining were performed to detect the transcripts of PUB19 in roots, stems, leaves, siliques and flowers. Both the distribution of GFP and cell fractionation assay displayed that PUB19 is a membrane-associated protein. All results showed some good findings to study the biological function of  PUB19 deeply.

Key words: PUB19, Expression pattern, Subcellular localization, Arobidopsis.