12483 10.30848/PJB2024-6(40) <p> A cytological study on the meiosis process of <em>Humulus scandens</em></p> Jinjin Guo, Yi Liang, Deqing Meng, Mengyi Wang, Yaning Liu, Geyang Wu, Jinhong Yuan and Junhua Li <p> <em>Humulus scandens</em> is a common dioecious weed that possesses valuable forage and medicinal properties. It exhibits strong adaptability and a high seed yield, making it a subject of significant research interest. However, there is a lack of information concerning the meiosis process of <em>H. scandens</em> and the relevant methods used for studying it. In this study, we aimed to address this gap by utilizing intact <em>H. scandens</em> meiosis cells as research material. We employed a rapid labeling method to observe the meiotic chromosomes. Additionally, a single oligonucleotide probe of 5S rDNA was used to asertain that the number of 5S rDNA signals in the somatic cells of <em>H. scandens</em> was one pair. Using this probe, we conducted an inspection of homologous chromosome pairing in <em>H. scandens</em>. Our study provides a comprehensive description of the meiosis process in <em>H. scandens</em> and introduces a set of cytological methods suitable for the easy labeling of meiotic chromosomes and the monitoring of homologous chromosome pairing in <em>H. scandens</em></p> Published 2024 56 6 2245 2250