12517 10.30848/PJB2025-2(34) <p> Comparison of antimicrobial, anticancer and antioxidant properties of Fe, Cu, Ag nanoparticles synthesized from <em>Salvia officinalis </em>plant</p> Esra Balkis, Murat Polat and Bulent Kaya <p style="margin-left:.25in;"> The goal of this study was to comparatively investigate the physicochemical and biological activities of nanoparticles (NPs) formed by the reduction of <em>Salvia officinalis </em>(<em>S. officinalis</em>) using iron (Fe), copper (Cu), silver (Ag) metal salts. Fe@NPs, Cu@NPs, Ag@NPs <em>S. officinalis</em> leaves were synthesized using silver nitrate (AgNO₃), copper (II) sulfate (CuSO₄), ferric chloride (FeCl₂), (FeCl₃). Physical-morphological properties of NPs were determined using instrumental analysis techniques FT-IR, UV-visible, SEM, TEM, EDX, XRD and Zeta potential analysis. The antibacterial activity of NPs was evaluated by the disk diffusion method. Total antioxidant, total flavonoid/phenolic capacity, DPPH scavenging activity, metal chelating activities of NPs were also examined. Cytotoxic activity of NPs on A549 lung cancer cell lines was determined <em>in-vitro</em> by WST-1 assay. Fe@NPs 270-290, Cu@NPs 270-320, Ag@NPs 300-400 nm generated strong surface plasmon resonance in the range. According to SEM/TEM results show that Fe@NPs are round-cubic 49.3; Global 30.24 of Cu@NPs; showed that Ag@NPs had circular dimensions of 5,36 nm. Zeta potential values were determined as Fe@NPs -22.2, Cu@NPs 25.5, Ag@NPs -24.6 mV. As a result of antimicrobial analysis, the highest effect was seen in Ag@NPs. Synergetic effects of the extract and metals were evaluated by determining the total antioxidant capacity and total flavonoid/phenolic content of <em>S. officinalis</em> and NPs. DPPH scanvenging activity, BHA (65.89%), <em>S. officinalis</em>, Fe@NPs, Cu@NPs, Ag@NPs; It was evaluated as 79.57%, 72.30%, 75.81%, 81.12%. Against the metal chelating feature of EDTA; <em>S. officinalis</em> was 42.92%, Fe@NPs, Cu@NPs, Ag@NPs were 16.75%, 18.02%, 15.13%. When the results of the anticancer analysis are evaluated; It was determined that Fe@NPs decreased cell viability by 90%, Cu@NPs by ~96% and Ag@NPs by 85%. This study reports the successful synthesis of Fe@NPs, Cu@NPs, Ag@NPs with <em>S. officinalis</em> leaf extract. The results show that Fe@NPs, Cu@NPs, Ag@NPs can be used in biomedical applications.</p> <p style="text-align: justify;"> &nbsp;</p> Published 2025 57 2 501 514