12892
10.30848/PJB2025-2(20)
<p>
Barcoding of<em> Chenopodium murale </em>L. with new record to Egyptian flora</p>
Amany S. Abdo, Wafaa M. Amer, Najla A. Al-Shaye and Walaa A. Hassan
<p>
<em>Chenopodium</em> L. is a cosmopolitan genus (family: Amaranthaceae). Hybridization and phenotypic plasticity of its species, complicate the species delimitation. The presence of observed notable phenotypic plasticity among the <em>C. murale</em> populations and the newly recorded (suspected <em>C. hybridum</em>), makes it challenging to identify these taxa's taxonomic identity. The morphological investigations revealed distinctive features at inter-specific level for <em>C. murale</em> populations mainly in stem color, leaf shape, margin dentation, number of stamens and tepals, and the percentages of both hermaphrodite flowers and the 5-staminode flowers. The morphological correlation between distinguished Forms; ranges between 0.070-0.518. While the infra-specific correlation between the <em>C. murale</em> forms and the suspected <em>C. hybridum</em> ranges from 0.033-0.340. RbcL and matK barcoding were applied to confirm the taxonomic identity as well as, their phylogenetic affinities of the studied taxa. The retrieved phylogenetic affinity between<em> C. murale</em> forms showed very low genetic divergence to the <em>C. hybridum</em>. The sequences of the confirmed <em>C. murale</em> forms and the new record to Egyptian flora<em> C. hybridum</em> were deposited in the world database under the accession numbers OR373113, OR394977, OR394978, OR394979, OR405949, OR405950, OR405951, and OR405952 for the <em>C. murale </em>and OR394980 and OR405953 for the <em>C. hybridum. </em>We investigated the inter-specific relationships among the <em>C. murale </em>forms, but the intra-specific relationships between <em>C. murale</em> and <em>C. hybridum </em>required further investigation to explore and fully understand the origin of this new record</p>
Published
2025
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