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OPTIMIZATION OF CULTURAL CONDITIONS FOR THE PRODUCTION OF L-DOPA (L-3, 4 DIHYDROXY PHENYL ALANINE) FROM L- TYROSINE BY ASPERGILLUS ORYZAE

IKRAM-UL-HAQ, SIKANDER ALl, SHUMAILA MANZOOR AND M.A. QADEER

Abstract: The present study is concerned with the optimization of different cultural conditions for the production of L-DOPA from L-Tyrosine by surface culture technique. Of the 12 different isolates of Aspergillus oryzae tested, for production of mycelium, strain ISB-9 was found to give maximum yield of L-DOPA (0.34 mg/ml) L-DOPA production was maximum at pH 3.0 of the reaction mixture, 50^oC, 50 minutes and 2.5 mg/ml, optimum temperature, time and L- Tyrosine concentration, respectively.

Biotechnology Research Laboratory, Department of Botany, Government College, Lahore, Pakistan.