| Pak. J. Bot., 35(1): 89-92, 2003. | Back to Contents | ||||
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Updated: 16-05-12 | ||||
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IN VITRO SELECTION FOR SALT TOLERANCE AMONG DIFFERENT STRAINS OF BRADYRHIZOBIUM JAPONICUM A. SHEREEN, R. ANSARI, S.M. ALAM, S. MUMTAZ AND S. RAZA Abstract: An experiment was conducted to study the comparative salt tolerance of 12 different strains of Bradyrhizobium japonicum (NIFTAL and USDA series) and strains isolated from locally grown soybean nodules collected from 3 different field locations under different salinity levels of sodium chloride (0, 25, 50, 75 and 100 mM NaCl corresponding to 0, 0.15, 0.29, 0.44 and 0.59 % NaCl, respectively). The rhizobium strains isolated from nodules were found to be highly sensitive when compared to the strains of NIFTAL and USDA series since there was a growth reduction of more than 80 % at the level of 50 mM NaCl. A wide range of variation was observed in the growth response of NIFTAL and USDA series with comparatively less reduction at the level of 50 mM NaCl. The strains TAL-950, TAL-102, TAL-103, TAL-377 were found comparatively salt tolerant. Nuclear Institute of Agriculture, Tandojam, Pakistan. |
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