| Pak. J. Bot., 39(3): 799-805, 2007. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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EFFECT OF GROWTH REGULATORS AND AMINO ACIDS
ON ARTEMISININ PRODUCTION IN THE CALLUS
OF ARTEMISIA
ABSINTHIUM
MUHAMMAD ZIA, ABDUL-MANNAN AND M. FAYYAZ
CHAUDHARY*
Abstract:
Studies
were conducted on the effect of amino acids and growth regulators on the
production of artemisinin, an antimalarial compound, in the callus of Artemisia absinthium. Callus was initiated on solid MS medium
supplemented with BAP and NAA from leaf explant. For the production of
artemisinin, the callus was proliferated on sterile filter paper bridge
in MS medium supplemented with different concentrations of plant growth
regulators and amino acids. Estimation of artemisinin contents showed
that leaves contained 223 μg/g artemisinin while no artemisinin was
observed in the stem extract. Callus culture initiated from leaf explant
on MS medium without any growth regulator, failed to show the presence
of artemisinin. The amount of artemisinin in the callus culture was
influenced with the addition of different growth regulators and amino
acids to the medium; 3.1 μg/g artemisinin was present in the callus
cultured on MS medium to which valine (12.5 mg/l) was added. Addition of
cystine (12.5 mg/l) to the medium resulted in 2.8 μg/g artemisinin
production. The amount of artemisinin in the callus cultures was 3.05μg/g
and 1.95 μg/g when BAP (2 mg/l) and NAA (2 mg/l), respectively were
present in the medium. Addition of other growth regulators and amino
acids resulted in nominal or no artemisinin production. Present study
suggest that artimisinin production can be enhanced with the
manipulation of medium by different hormones and amino acids in the
callus cultured on sterile filter paper bridge.
Department
of Microbiology, Corresponding Author's E-mail: fayyazchaudhary@yahoo.com |
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