|
|
|
EFFECT OF VARIETY AND PLANT
GROWTH REGULATORS ON CALLUS PROLIFERATION AND REGENERATION RESPONSE OF
THREE TOMATO CULTIVARS
(LYCOPERSICON ESCULENTUM)
ZUBEDA
CHAUDHRY, AMBER AFROZ AND HAMID RASHID*
Abstract:
The
experiment was conducted to optimize a reproducible protocol for callus
induction and regeneration of three tomato cultivars and also to select
the cultivar which better perform under In
vitro conditions for further experimentation. For callus induction
hypocotyls and leaf discs were used as explant source. Explants of the
tomato seedlings were cultured on MS medium supplemented with different
concentrations and combinations of different plant growth regulators (PGRS)
for callus proliferation. Callus induction values were significantly
influenced by the variety and explant source. In all three tested
cultivars maximum callus induction frequency was observed on CIM6 (MS +
0.5 mg/l IAA+2mg/l 2ip). There was also a positive correlation between
the treatment and explant source. Here at
T6 (2ip 2mg/l,
IAA0.5 mg/l) hypocotyls gave its maximum value of (81.8%), followed by theT4 (IAA 1mg/l+kinetin
0.5 mg/l) for hypocotyls (79.48%). To observe the regeneration capacity
of three tomato cultivars, 10 Regeneration Media (RM) combinations were
tested. The regeneration capacity is significantly influenced by
cultivar and explant type. The best regeneration media was found to be
RM3 (MS +1.5 mg/l 2ip and 0.5 mg/L IAA) with (72.5-79.22%) regeneration.
Among varieties Riogarande showed maximum regeneration capacity
(79.22%).
Agricultural
Biotechnology Programme (ABP), Institute of Agricultural Biotechnology
& Genetic Resources (IABGR), National Agricultural Research Center (NARC),
Park
Road, Islamabad, Pakistan
*Corresponding Author's E-mail: Ph #:
9251-9255217, Fax #: 9251-9255217,
bi_narc@yahoo.com
|
|
|