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KINETICS
OF INVERTASE PRODUCTION BY SACCHAROMYCES CEREVISIAE
IN BATCH CULTURE
IKRAM-UL-HAQ AND SIKANDER ALI
Abstract:
The
present study is concerned with the improved invertase production by Saccharomyces
cerevisiae on kinetic basis. The wild-culture (IIB-1) and putative
mutant (NA6) of S. cerevisiae were compared on time course basis
when grown in batch culture. The values
for Yx/s, Yp/s and Yp/x with urea were
significantly improved (p≤0.05) over the control (peptone+yeast
extract) as well as peptone supplementation. The maximum growth in terms
of volumetric rate for cell mass formation (Qx), 48 h after
the incubation was only marginally different from control. The
values of both Km
and Vmax of the invertase from mutant culture were
significantly improved, as were the specificity constant and kcat
for the enzyme. The activation energy
and substrate binding for sucrose fermenting were found to be highly
significant for yeast culturing.
Institute
of Industrial Biotechnology, GC University, Lahore, Pakistan
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