|
|
|
NODULATION
OF
SESBANIA
SPP.,
BY INTRODUCED RHIZOBIA IN COMPETITION WITH NATURALIZED
STRAINS
IN DIFFERENT SOIL TYPES
FAUZIA Y. HAFEEZ*, **FARRUKH
NAEEM**, NAZIA SHAHEEN
AND KAUSAR A. MALIK
Abstract:
Seven rhizobial strains were
isolated from
Sesbania
aculeata
and three from
Sesbania grandifolia
from three different soil
types. These strains were characterized for their morphology and carbon
source utilization. The affectivity of these strains was confirmed by re
inoculating the host and were effective on both
Sesbania
spp. The strains Sa1 and Sa2,
however found to be more effective having nitrogenase activity ranging
from 3.7-85.2 and from 4.8-77.7 m moles of C2H4 produced
h-1 g-1 nodule dry weight respectively. The
consortium of Sa1, Sa2, Sg1 and Sg2 was also found to be highly
effective in both
Sesbania
spp.
In competition with the indigenous population, the strains Sa1 and Sa2
were found to be most competitive among the others. Fluorescent antibody
studies showed the nodule occupancy ranged from 30-100% by Sa1 and Sa2
in both species. Response of inoculum was better in soils 1 & 2
which were low in nutrient level than in soil 3.
National Institute for Biotechnology and Genetic
Engineering
P.O. Box 577 Jhang Road, Faisalabad, Pakistan.
**Present address: Department of Mycology
and Plant Pathology,
University of the Punjab, Lahore.
|
|
|