| Pak. J. Bot., 39(5): 1787-1795, 2007. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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INDUCTION AND REGENERATION OF HYPOCOTYL DERIVED CALLI IN HOT CHILLI (CAPSICUM FRUTESCENS L.) VARIETIES RABBIA HASNAT, NADEEM AKHTAR ABBASI, TOUQEER AHMAD AND ISHFAQ AHMAD HAFIZ Abstract: An efficient and reliable In vitro protocol was established for induction and regeneration of calli in varieties of hot chilli (Capsicum frutescens L.) var. Nepali and NARC-IV. Early initiation and induction with sustainable calli growth in both varieties was achieved on Murashige & Skoog medium supplemented with 1.5 mg l-1 of 2,4-D. Better performance in both varieties regarding shoot initiation, regeneration rate (%) and number of lateral shoots per regenerants were achieved on medium containing 3.0 mg l-1 BA. Rooting was achieved on half strength MS basal medium containing 1.0 mg l-1 IBA. Plantlets exhibiting normal morphology with extensive rooting were acclimatized in greenhouse.
Department of Horticulture, University of Arid Agriculture,
Rawalpindi, 46300, Pakistan. |
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