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MOLECULAR AND BIOCHEMICAL ASSESSMENT OF BRASSICA NAPUS AND INDIGENOUS CAMPESTRIS SPECIES

S. J. ABBAS­­¹, FARHATULLAH², I. A. KHAN¹, KHAN BAHADAR MARWAT³ AND IQBAL MUNIR¹

Abstract: In Pakistan, Brassica is the second most important source of oil after cotton. It contributes about 17% to the domestic production of edible oil. Parental lines along with five F₂s were assessed for biochemical parameters using Near Infrared Reflectance Spectroscopy (NIRS). Parental lines contain more oil 45.85% as compared to F₂s 42.26% while the F₂s contain more protein 25.92% as compared to the parents 23.70%. Both parents and F₂ contain high glucosinolate and fatty acids contents. Insulin Growth like Factor (IGF) primer sets were used to estimate genetic relationship among 5 F₂ segregating population of Brassica along with 9 parental lines. On an average 29 alleles were amplified using IGF primer sets.  Mean genetic distance estimates ranged from 0.25-1.00 (G.D = 0%-100 %), respectively.  Size of scorable fragments ranged from approximately 250 to >2000 bp.  A high level of genetic dissimilarity (GD= up to 100%) was estimated among all genotypes.  Entries were grouped in clusters using cluster analysis.  On the basis of dendrogram, most diverse genotypes were identified which should be utilized in further breeding programs aimed at creating genetic variability in local germplasm.

¹Institute of Biotechnology & Genetic Engineering, NWF Agricultural University, Peshawar, Pakistan.

²Department of Plant Breeding and Genetics, NWF Agricultural University, Peshawar, Pakistan.

³Department of Weed Science, NWFP Agricultural University, Peshawar, Pakistan.