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PHENOLIC CONTENT IN VITRO CULTURES 0F CHICK PEA (CICER ARIETINUM L.) DURING CALLOGENESIS AND ORGANOGENESIS

SHAGUFTA NAZ¹, AAMIR ALI² AND JAVED IQBAL³

Abstract: The callogeneic response of different explants i.e. cotyledon, leaf, node, shoot and root apices of Cicer arietinum was investigated on MS basal media supplemented with different concentrations of auxins and cytokinins. A combination of BAP and 2,4-D or NAA enhanced proliferation response. Of different combinations MS+1.0 2,4-D+0.5 NAA+0.5 BAP mg/l proved exceptionally good both for callus induction and proliferation. Callus masses initiated from different explants were maintained from main to 10^thsub cultures. The morphogenic behaviour of different calli was also investigated. It was observed that the rate of browning and necrosis was increased with the increasing subcultures. To elucidate the biochemical basis of growth inhibition in non-morphogenic and morphogenic calli, total phenolics were estimated from main to 10^th sub cultures both quantitatively and qualitatively. A gradual increase in total phenolic content was noticed from main to 10^th sub cultures in both non-morphogenic and morphogenic calli. Non-morphogenic calli exhibited higher phenolic content as compared to morphogenic calli. The qualitative analysis of phenolic compound was also in conformity with quantitative data as the number of phenolic spots increased from main to 10^th subcultures.

1Department of Botany, Lahore College for Women University, Lahore, Pakistan

2Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan

³School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan