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STUDY OF GENETIC DIVERSITY IN WHEAT (TRITICUM AESTIVUM L.) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

SAJIDA BIBI, M. UMAR DAHOT¹, IMTIAZ A. KHAN, A. KHATRI AND M.H. NAQVI²

Abstract: Twelve wheat genotypes developed through hybridization programme were screened for genetic diversity through RAPD marker. A total of 102 loci were amplified with 14 primers out of which 91 (89.2%) were polymorphic and only 11(10.8%) were monomorphic. Fragments size ranged from 142bp-5.3kb and fragments produced by various primers ranged from 1-11 with an average of 7.1 fragments per primer. The highest number of loci (13) was amplified with primer A-10, while the lowest number (1) with primer B-10. Results revealed that variety SARC-1, PKV-1600 and Chakwal-86 contain a specific segment of 478 bp while SARC-1 contains another specific segment of 957 bp amplified with primer A-07. Genetically most similar genotypes were SARC-1 and Chakwal-86 (70%) while most dissimilar genotypes were Sarsabz and PKV-1600 (33%). On the basis of results achieved, the varieties could be divided into 3 groups, Kiran-95, Marvi-2000 and Sarsabz in one group, Bhitai, ESW-9525, Inqilab-91, Khirman and Abadgar-93 clustered in second group and SARC-1, Chakwal-86, PKV-1600 and CM 24/87 in the third group.

Agriculture Biotechnology Division, Nuclear Institute of Agriculture, Tando jam, Pakistan

¹Institute of Biotechnology and Genetic Engineering (IBGE), University of Sindh, Jamshoro, Pakistan.

²Pakistan Atomic Energy Commission, Islamabad, Pakistan
E-mail: niatjam@hyd.pak.net.com.pk; E-mail: sajidakhanmughal79@hotmail.com