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DEVELOPMENT OF GENETIC LINKAGE MAP OF LEAF RED COLOUR IN COTTON (GOSSYPIUM HIRSUTUM) USING DNA MARKERS

IFTIKHAR ALI¹, MUHAMMAD ASHRAF^*, MEHBOOB-UR-REHMAN^**, YUSUF ZAFAR^**, MUHAMMAD ASIF^**, ABIDA KAUSAR, SANA RIAZ, MUBASHIR NIAZ, ABDUL WAHID^* AND SYED QAISER ABBAS

Abstract: Red leaf colour in cotton (Gossypium hirsutum L.) variety SL 7-9 and green leaf colour variety FH-634 was crossed to raise F₂ and F₃ segregating populations for RAPD and SSR analysis. A total of 400 RAPD and 54 SSR primers were used to find out DNA markers linked with the red leaf colour. Two RAPD primers (OPL-09 and OPZ-11) and three SSR primer pairs CM-43, CM-162 and JESPR-204 amplified polymorphic DNA fragments for leaf colour trait. These findings provide basic information about the inheritance of red colour in cotton using RAPD and SSR for linkage analysis and the construction of genetic linkage map. The value of framework markers will be enhanced if locus information (fragment sizes, restriction enzymes, primers, etc.) is fully explored in each map.

Department of Botany, GC University, Faisalabad, Pakistan

*Department of Botany, University of Agriculture, Faisalabad, Pakistan

**National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan
Corresponding author's email: Iftikhar_pbd@yahoo.com