| Pak. J. Bot., 41(3): 1163-1171, 2009. | Back to Contents | ||||
|
|
Updated: 09-07-09 | ||||
|
ANALYSIS OF THE GENETIC RELATIONSHIP BETWEEN PIPER METHYSTICUM AND PEPPER BY AFLP
JIANG SHIA, LI XIN1, YAN YANG2, XUEQIN ZHENG3*, XIANGSHENG KONG1, ZHIYONG LI1, XIAOLING WANG1, SHUANGCHENG GAO1
Abstract: The aim of the research was to discuss
the genetic relationships among Piper methysticum, Pepper and it
wild relatives. With 28 germplasms including 6 materials of P.
methysticum, 21 materials of cultivated and wild pepper, 1 material
of Peperomia pellucida belonged to different genus as tested
materials, DNA was extracted from leaves and primers with good band-type
and high polymorphism and resolving power were screened from 64 pairs of
primers for AFLP amplification and the clustering analysis was conducted
with MVSP3.13f software. 191 bands were amplified by 4 pairs of primers,
189 of which had polymorphism being 98.6%. 28 germplasms were clustered
into 6 different groups at the genetic similarity coefficient of 0.52 by
silver staining AFLP, in which 6 materials of Piper methysticum
were clustered into a single group, indicating that P. methysticum
belonged to Pipper
L., Piperaceae
and were distantly related to the others. The research provided the
basis for selecting rootstocks, molecular identification and the
fingerprint construction of P. methysticum. 1College of Agriculture, Henan University of Science and Technology, Tianjin Road 70, Jianxi District, Luoyang Henan 471003, China 2Beijing Dingguo Biotechnology Co., Ltd., Xueyuannanlu 32, Haidian District, Beijing 100088 China
3State Key Biotechnology Laboratory for Tropical Crops, Chinese Academy of
Tropical Agricultural Sciences, Xueyuanlu, Chengxi District, Haikou
Hainan 571101 China |
|||||
|
|
|||||
| Back to Contents |
|
Back to Contents | |||
|
|
|||||