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Pak. J. Bot., 42(3): 1857-1862, 2010. |
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Updated: 07-12-10 | ||||
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HIGH FREQUENCY REGENERATION SYSTEM OPTIMIZATION FOR WHEAT CULTIVAR INQILAB-91
ASMA AFZAL1, HAMID RASHID2*, M. HAROON KHAN2, ZUBEDA CHAUDHRY3 AND SALMAN A. MALIK1
Abstract: In the present study, a high efficiency regeneration system was developed in wheat (Triticum aestivum L.) cv. Inqilab-91. Mature embryos were taken as explant source and inoculated on MS media for callus induction. MS media with different concentrations of 2, 4-D (2mg/l, 3mg/l and 4mg/l) were used for callus induction. Maximum percentage (76.04%) of callus induction was achieved with 3mg/l of 2, 4-D whereas 53.12% and 56.25% of calli were formed with 2mg/l and 4mg/l of 2, 4-D respectively. Four different hormones (IAA, BAP, Kn and 2ip) were used in different concentrations and combinations in MS medium for regeneration from calli of mature embryos. The regeneration frequency (46.66%) was obtained with 0.1mg/l of IAA and 0.5mg/l of BAP. Different concentrations of Kn (0mg/l, 0.5mg/l, 1.0mg/l) were added to the optimized concentration of IAA (0.1mg/l) and BAP (0.5mg/l) to achieve more regeneration frequency. Regeneration frequency (56.66%) was obtained with 0.5mg/l of Kn along with IAA (0.1mg/l) and BAP (0.5mg/l). 60% regeneration was achieved on medium containing IAA 0.1mg/l and Kn 0.5mg/l. Different concentrations of 2ip (0mg/l, 0.5mg/l, 1.0mg/l and 1.5mg/l) were also tested along with IAA and Kn and regeneration frequency of 65% with IAA (0.1mg/l), Kn (0.5mg/l) and 2ip (1.0mg/l) which was maximum in all the combinations experimented before. High frequency system ranged from 46.66 to 65% depending upon the type of hormone concentration used was optimized. We were successful to achieve high frequency regeneration by manipulating hormonal type and concentration which were not reported earlier.
1Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan 2Department of Bioinformatics, Mohammad Ali Jinnah University, Islamabad 3Department of Botany, Haraza University, Mansehra. |
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