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DIVERSITY OF GLUTELIN ΑLPHA SUBUNITS IN RICE VARIETIES FROM PAKISTAN

NADAR KHAN¹*, NAUSHAD ALI², MALIK ASHIQ RABBANI¹, MUHAMMAD SHAHID MASOOD¹

Abstract: The diversity of glutelin α subunits in thirty-two rice varieties from Pakistan was assessed using higher temperature sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Five variation types i.e. Type-I, Type-II, Type-III, Type-IV and Type-V on the basis of variation in molecular size and intensities of glutelin α subunits were identified. Six rice varieties, i.e., Khushboo-95, Shahkar, Sonahri-Sugdasi, Sugdasi-Bengalo, Sugdasi-Ratria and Sugdasi-Sadagulab exhibited Type-IV variation having comparatively higher glutelin content. These varieties may be important genetic resources for improving glutelin content of rice seed. Rice variety Jhona-349 exhibited Type-V variation type and displayed unique variation by showing the presence of unique bands. Accordingly, this variety might have some unique glutelin genes and need to be further explored for nutritional quality improvement of rice seed.  

¹Institute of Agricultural Biotechnology and Genetic Resources (IABGR), National Agricultural Research Centre (NARC), Islamabad, Pakistan.

²Department of Plant Breeding and Genetics, NWFP Agricultural University, Peshawar, Pakistan.

*Corresponding author E-mail: nadarkhan@hotmail.com