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EFFECT OF A STRONG ENZYME DENATURANT (UREA) ON THE STABILITY OF SOLUBLE ACID INVERTASES FROM SUGARCANE

ALTAF HUSSAIN¹, ZAFAR IQBAL KHAN²*, KAFEEL AHMAD², MUHAMMAD ASHRAF^1,!, EHSAN ELAHI VALEEM³* AND MUHAMMAD HAMID RASHID⁴

Abstract: An investigation was performed to determine the effect of urea, a strong enzyme denaturant on the stability of invertases from two cultivars of sugarcane. The stability of native soluble acid invertases (SAI) of COJ-84 (local cultivar of sugarcane) to urea showed that they were more stable than those of CP-77-400 (local cultivar of sugarcane). The stability of SAI of CP-77-400 to urea gave biphasic trend at different salinity levels, and the stability at these levels decreased significantly as compared to control. The stability of 50 mM salinity level SAI was also decreased, while at 150 mM salinity level remained unchanged. The SAI of COJ-84 secreted under saline growth media presented extreme stability to unfolding by urea. The SAI at 150 and 200 mM salinity levels gave an activation trend and did not show denaturation.

¹Department of Botany, University of Agriculture, Faisalabad-38040, Pakistan.

²Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan.

³Department of Botany, Govt. Degree College Buffer Zone, Karachi-75850, Pakistan.

⁴ National Institute of Biotechnology and Genetic Engineering, Faisalabad, Pakistan. !Second affiliation: King Saud University, Riyadh, Saudi Arabia.

*Corresponding Authors: drzafar10@hotmail.com; valeem@hotmail.com