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Updated: 04-07-13

A NEW METHOD FOR THE ISOLATION AND PURIFICATION OF LAWSONE FROM LAWSONIA INERMIS AND ITS ROS INHIBITORY ACTIVITY

SYED MUHAMMAD GHUFRAN SAEED^1*, SYED ASAD SAYEED¹, SEEMA ASHRAF¹, SHAHINA NAZ¹, RAHMANULLAH SIDDIQI¹, RASHIDA ALI^{1, 2, 4} AND M. AHMED MESAIK³

Abstract: Lawsone a coloring component of the Henna (Lawsonia inermis) leaves was found to bind with proteins. Present study has suggested a new rapid method for the isolation of lawsone from L. inermis through the calcium ion using the Flash and Disc Counter Current Chromatography (DCC). The purified pigment structurally characterized by 2D, TLC and NMR spectroscopy, was found to be active against reactive oxygen species (ROS) by Chemeluminiscence assay using lymphocytes. Purified lawsone showed remarkable inhibitory activity on the oxidative burst response of the whole blood, polymorphonuclear cells (PMNCs), Mononuclear cells (MNCs) with IC50 as 13 ±1.6 mg/ml, 11±2.7 mg/ml. and 10±4.2 mg/ml respectively.
 

¹Department of Food Science and Technology, University of Karachi, Karachi, 75270, Pakistan

²Division of Food Research, HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan

³Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan

⁴English Biscuit Manufacturers (Pvt.) Limited, Plot 1-4, Sector 23, Industrial Area, Karachi-749 00, Pakistan.

^*Corresponding author’s e-mail: sas60pk@yahoo.co.uk, smghufransaeed@yahoo.com