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Pak. J. Bot., 45(S1): 347-352, 2013.

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INSILICO IDENTIFICATION OF ALTERNATE RESISTANCE GENE AGAINST YELLOW RUST IN WHEAT

SHAHZADI FAIZA SAFDAR¹, HAMID RASHID¹*AND ZUBEDA CHAUDHRY²

Abstract: Yellow rust, also known as stripe rust, is one of the 3 wheat rust diseases principally found in wheat grown in cooler environments. Yellow rust, grounds by Puccinia striiformis Westend. f.sp. tritici, which is an important foliar disease of wheat and development resistant cultivars are the most economical method of control. Of the three rust diseases of wheat, stripe (yellow) rust (YR) is the most damaging to grain yields in cool, moist environments. Chemical control of rusts is expensive and hazardous to the environment. Yr5 and Yr10 are resistance genes among many other resistance genes while PsMAPK1 is fungus protein causing yellow rust in wheat. The protein sequences of Yr5 and Yr10 was retrieved from NCBI and PsMAPK1 protein sequence was retrieved from UNIPROT. By I-TASSER 3D models of Yr5, Yr10 and PsMAPK1 were generated. The models were evaluated by energy minimization technique. By finding the physiochemical properties of the templates and by docking technique alternate resistance gene had been found from the Yr5 and Yr10 templates. The physiochemical properties of Yr5 and Yr10 genes were compared with physiochemical properties of templates to find the suitable alternate against this disease in wheat. Docking results shows the interactions among receptor and ligand. These results show that template 2OF7 has closer results of physiochemical properties to Yr5 and template 3IZA has closer results of physiochemical properties to Yr10.  

¹Department of Bioinformatics, Mohammad Ali Jinnah University, Islamabad, Pakistan

²Department of Botany, Hazara University, Mansehra, Pakistan

^*Corresponding author’s e-mail: drhamid@jinnah.edu.pk