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Pak. J. Bot., 47(5): 2277-2282, 2015. |
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Updated: 02-01-16 | ||||
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In silico based development of dLUTE length polymorphism marker for common flax germplasm evaluation
JANA ŽIAROVSKÁ*, MILAN BEŽO, VERONIKA LANCÍKOVÁ AND KATARÍNA RAŽNÁ
Slovak University of Agriculture in Nitra, Department of Genetics and Plant Breeding, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovak Republic *Corresponding author e-mail: jana.ziarovska@uniag.sk; Ph: +421376414244; fax +421376415599
Abstract: The first study of the pattern of dLUTE insertions as detected by PCR length polymorphism technique was performed. In silico approach was used for developing the dLUTE based marker system. Alignment analysis were performed to find a region that is dLUTE specific and primers designed withing this region not corresponding to any of sequences of L-genes. Realized length polymorphism analysis of common flax accessions confirmed the results of In silico analysis of polymorphic potential of designed marker system. The PCR analyse based of flax dLUTE transposon resulted in ten different alleles amplified in PCR withing the length range from 250 up to the 980 bp. In total, twenty-four common flax accessions were evaluated using the developed approach and the grouping of them according the oil/fiber type was obtained in constructed dendrogram.
Key words: Linum usitatissimum, L.; dLUTE; Length polymorphism; DNA marker.
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