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Pak. J. Bot., 47(6): 2335-2338, 2015.

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GENETIC VARIATIONS OF ROBINIA PSEUDOACACIA PLANT USING SDS-PAGE

MUHAMMAD ZAHOOR¹, MUHAMMAD NISAR² AND NOOR UL ISLAM¹

¹Department of Chemistry, University of Malakand, Chakdara Dir (Lower) Khyber Pakhtunkhwa Pakistan

²Department of Botany, University of Malakand, Chakdara Dir (Lower) Khyber Pakhtunkhwa Pakistan

^*Corresponding author e-mail: zahoorshabab@gmail.com

Abstract: The biochemical analysis using SDS-PAGE has great contribution for the estimation of genetic diversity. We estimated the genetic diversity of R. pseudoacacia germ plasm protein. A total of 19 varieties were collected from different areas of Dir lower were investigated for the level of genetic divergence and genetic linkages. The total germ plasm grouped were separated at 20% distance into two linkages based on Euclidean distances the 19 cultivars were further divide at 45% distance into three clusters, cluster I, cluster 2 and cluster 3. Cluster 1 was comprised of Munda 3, Munda 4, Talash 2 and UOM 1. Cluster 2 was comprised of Maidan 1 and Gulabad 1. Cluster 3 was comprised Maidan 2, UOM 3, Talash 1, Maidan 4, Maidan 3, Gulabad 2, Gulabad 3 and Gulabad 4. A total of range 00% to 88% variation recoded among 19 varieties. . The result obtained after SDS-PAGE were computed for the construction of phylogenetic diversity, geographic relationship, Euclidian distance, genetic distance and linkage distance .This plant show a lot of variation in germ plasmic level. It is concluded that it is possible to improve and produce new varieties of this plant.

Key words: Robinia pseudoaacacia, SDS-PAGE, Polygenetic diversity.