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Pak. J. Bot., 47(6): 2355-2359, 2015. |
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Updated: 02-01-16 | ||||
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DEVELOPMENT OF HIGH EFFICIENCY MICROPROPAGATION PROTOCOL FOR TAMARIX NILOTICA EHRENB WITH VALUED MEDICINAL PROPERTIES
FAHAD AL-QURAINY, MOHAMMAD NADEEM*, SALIM KHAN, MOHAMED TARROUM AND SALEH ALANSI
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia *Corresponding author’s e-mail: mohammadnadeem911@hotmail.com; Tel.: +966-01467-5876; Fax: +966-467-8301.
Abstract: Tamarix nilotica is an important medicinal plant grows throughout the Kingdom, except in mountains on high altitude. Propagation of T. nilotica through cuttings and seeds are limited. To supplement harvesting of active ingredient from plants, alternative method for the purpose has been developed. Stem node cuttings were subjected to in vitro culture under the influence of various cytokinin to induce shoot proliferation. Maximum shoot stimulation was found on MS medium comprising 1.0 µM TDZ followed by 2.5 µM BA, Kn and 2ip. The number of shoots declined as the concentration of cytokinin increased. A lower shoots obtained on 5.0 and 10.0 µM concentration of all the four cytokinins tested. After fair multiplication, individual shoots were subjected to different concentration of auxins IBA, NAA, 2,4-D and IAA 2.5-10.0 µM for root induction. Initial screening did not result desired rooting on MS medium. Therefore, pulse treatment of 100 µM IBA was given to the isolated shoots for 5, 10 and 15 days time. Incubation for 10 days on MS medium supplied with 100.0 µM resulted in maximum rooting when transferred on MS medium alone. Well rooted microshoots were exposed to four types of soil mixtures for acclimatization of plants. Among these soils, sand and FYM gave 100% survival under controlled green house conditions. This protocol would be helpful in regeneration and conservation of this plant species; and provide an alternative source of biomass for pharmaceutical active ingredients.
Key words: Micropropagation, Tamarix nilotica, Tissue culture, Medicinal plant.
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